Dipl.-Biol. Siegfried J.W. Ruppert

U.S. Attorney | U.S. Patent Attorney

Scientific Publications

During his scientific career Dr. Ruppert was involved in the research projects described in the publications listed below:

  • Shao, Z., Siegert, J.L., Ruppert, S. and Robbins, P.D. (1997). Rb interacts with TAFII250/TFIID through multiple domains distinct from E2F and viral oncoprotein binding regions.  Oncogene 15, 385-392.
  • Marsters, S.A., Pitti, R.M., Donahue, C.J., Ruppert, S., Roberts, P., Bauer, K.D. and Ashkenazi, A. (1996). Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA.  Current Biology 6, 750-752.
  • Pitti, R.M., Marsters, S.A., Ruppert, S., Donahue, C.J., Moore, A. and Ashkenazi, A. (1996). Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 271, 12687-12690.
  • Dikstein, R., Ruppert, S. and Tjian, R. (1996). TAFII250 is a bipartite protein kinase that phosphorylates the basal transcription factor RAP74.  Cell 84, 781-790.
  • Ruppert, S. and Tjian, R. (1995). Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation. Genes & Dev. 9, 2747-2755.
  • Shao, Z., Ruppert, S. and Robbins, P.D. (1995). The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250. Proc. Natl. Acad. Sci. USA 92, 3115-3119.
  • Tansey, W.P., Ruppert, S., Tjian, R. and Herr, W. (1994). Multiple regions of TBP participate in the response to transcriptional activators in vivo. Genes & Dev. 8, 2756-2769.
  • Kelsey, G., Ruppert, S., Beermann, F., Grund, C., Tanguay, R.M. and Schütz, G. (1993). Rescue of mice homozygous for lethal albino deletions: Implications for an animal model for human liver disease tyrosinaemia type 1. Genes & Dev. 7 (12a), 2285-2297.
  • Shelly, L.L., Lei, K.-J., Pan, C.-J., Sakata, S.F., Ruppert, S., Schütz, G. and Chou, J.Y. (1993). Isolation of the gene for murine glucose-6-phosphatase, the enzyme deficient in glycogen storage disease Type 1A.  J. Biol. Chem. 268, 21482-21485.
  • Weinzierl, R.O.J., Ruppert, S., Dynlacht, B.D., Tanese, N. and Tjian, R. (1993). Cloning and expression of Drosophila TAFII60 and human TAFII70 reveals conserved interactions with other subunits of TFIID. EMBO Journal 12 (13), 5303-5309.
  • Ruppert, S., Wang, E.H. and Tjian, R. (1993). Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature 362, 175-179.
  • Sakata, S.F., Shelly, L.L. Ruppert, S., Schütz, G. and Chou, J.Y. (1993). Cloning and expression of murine S-adenosylmethionine synthetase. J. Biol. Chem. 268, 13978-13986.
  • Beermann, F., Schedl, A., Ruppert, S., Ganss, R., Schmid, E., Klueppel, M., Hummler, E. and Schütz, G. (1993). The mouse tyrosinase gene: Pigment cell-specific gene expression in transgenic mice. In: Pharmacology of the Skin, Vol. 5, From Molecular Biology to Therapeutics, B.A. Bernhard and B. Shroot (eds), Karger, Basel, 57-62.
  • Kelsey, G., Ruppert, S., Schedl, A., Schmid, E., Thies, E. and Schütz, G. (1992). Multiple effects on liver-specific gene expression in albino lethal mice caused by deficiency of an enzyme in tyrosine metabolism. Journal of Cell Science – Supplement 16, 117-122.
  • Rosewicz, S., Vogt, D., Harth, N., Grund, C., Franke, W.W., Ruppert, S., Schweitzer, E., Riecken, E.O. and Wiedenmann, B. (1992). An amphicrine pancreatic cell line: AR42J cells combine exocrine and neuroendocrine properties. European Journal of Cell Biology 59, 80-91.
  • Beermann, F., Schmid, E., Ganss, R., Schütz, G. and Ruppert, S. (1992). Molecular characterization of the mouse tyrosinase gene: Pigment cell-specific expression in transgenic mice. Pigment Cell Research 5, 295-9.
  • Ruppert, S., Kelsey, G., Schedl, A., Schmid, E., Thies, E. and Schütz, G. (1992). Deficiency of an enzyme of tyrosine metabolism underlies altered gene expression in newborn liver of lethal albino mice.  Genes & Dev. 6, 1430-1443.
  • Schedl, A., Ruppert, S., Kelsey, G., Thies, E., Niswander, L., Magnuson, T., Klebig, M.L., Rinchik, E.M. and Schütz, G. (1992). Chromosome jumping from flanking markers defines the minimal region for alf/hsdr-1 within the albino-deletion complex. Genomics 14, 288-297.
  • Kelsey, G., Schedl, A., Ruppert, S., Klebig, M.L., Niswander, L., Magnuson, T., Rinchik, E.M. and Schütz, G. (1992). Physical mapping of the albino-deletion complex in the mouse to localize alf/hsdr-1, a locus required for neonatal survival. Genomics 14, 275-287.
  • Cole, T., Copeland, N.G., Gilbert, D.J., Jenkins, N.A., Schütz, G. and Ruppert, S. (1992). The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization. Genomics 13 (4), 974-982.
  • Ruppert, S., Cole, T.J., Boshart, M., Schmid, E. and Schütz, G. (1992). Multiple mRNA isoforms of the transcription activator protein CREB: generation by alternative splicing and specific expression in spermatocytes. EMBO Journal 11, 1503-1512.
  • Sharan, S.K., Holdener-Kenny, B., Ruppert, S., Schedl, A., Kelsey, G., Rinchik, E.M. and Magnuson, T.  (1991). The albino-deletion complex of the mouse: molecular mapping of deletion breakpoints that define regions necessary for development of the embryonic and extraembryonic ectoderm. Genetics 129, 825-832.
  • Beermann, F., Ruppert, S., Hummler, E. and Schütz, G. (1991). Tyrosinase as a marker for transgenic mice. Nucleic Acids Res. 19, 958.
  • Klüppel, M., Beermann, F., Ruppert, S., Schmid, E., Hummler, E. and Schütz, G. (1991). The mouse tyrosinase promoter is sufficient for expression in melanocytes and in the pigmented epithelium of the retina. Proc. Natl. Acad. Sci. USA 88, 3777-3781.
  • Chou, J.Y., Ruppert, S., Shelly, L.L. and Pan, C.-J. (1991). Isolation and characterization of mouse hepatocyte lines carrying a lethal albino deletion. J. Biol. Chem. 266, 5716-5722.
  • Niswander, L., Kelsey, G., Schedl, A., Ruppert, S., Sharan, S.K., Holdener-Kenny, B., Rinchik, E.M., Edström, J.-E. & Magnuson, T. (1991). Molecular mapping of albino deletions associated with early embryonic lethality in the mouse. Genomics 9, 162-169.
  • Nitsch, D., Ruppert, S., Kelsey, G., Schedl, A., Weih, F., Stewart, F., Strähle, U., Schmid, W., deVack, C., Reik, A., Boshart, M. and Schütz, G. (1991). Hormonal and liver-specific control of expression of the tyrosine aminotransferase gene. In: Molecular Aspects of Cellular Regulation. Vol. 6 The Hormonal Control Regulation of Gene Transcription. p 223-234. P. Cohen and J.G. Foulkes (eds). Elsevier Science. Publishers B.V. Amsterdam, The Netherlands.
  • Beermann, F., Ruppert, S., Hummler, E., Bosch, F.X., Müller, G., Rüther, U. and Schütz, G. (1990). Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice. EMBO Journal9, 2819-2826.
  • Schöler, H.R., Ruppert, S., Suzuki, N., Chowdhury, K. and Gruss, P. (1990). New type of POU domain in germ line-specific protein Oct-4. Nature 344, 435-439.
  • Ruppert, S., Boshart, M., Bosch, F.X., Schmid, W., Fournier, K. and Schütz. G. (1990). Two genetically defined trans-acting loci coordinately regulate overlapping sets of liver-specific genes.  Cell 61, 895-904.
  • Ryseck, R.-P., Macdonald-Bravo, H., Mattéi, M.-G., Ruppert, S. and Bravo, R. (1989). Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor. EMBO Journal 11, 3327-3335.
  • Kelsey, G.D., Ruppert, S., Boshart, M., Schedl, A., Schmid, W. and Schütz, G. (1989). The albino perinatal lethal deletion: Identification of affected mRNAs and mapping of the locus by pulsed-field gel electrophoresis.  NATO ASI Series, Vol. H34. Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation. pp 47-62.
  • Müller, M.M., Ruppert, S., Schaffner, W. and Matthias, P. (1988). A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.  Nature 336, 544-551.
  • Müller, G., Ruppert, S., Schmid, E. and Schütz, G. (1988). Functional analysis of alternatively spliced tyrosinase gene transcripts. EMBO Journal 9, 2723-2730.
  • Ruppert, S., Müller, G., Kwon, B. and Schütz, G. (1988). Multiple transcripts of the mouse tyrosinase gene are generated by alternative splicing. EMBO Journal9, 2715-2722.
  • Becker, P.B., Ruppert, S. and Schütz, G. (1987). Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors. Cell 51, 435-443.
  • Müller, G., Scherer, G., Zentgraf, H., Ruppert, S., Herrmann, B., Lehrach, H. and Schütz, G. (1985). Isolation, characterization and chromosomal mapping of the mouse tyrosine aminotransferase gene. J. Mol. Biol. 184, 367-373.
  • Ruppert, S., Scherer, G. and Schütz, G. (1984). Recent gene conversion involving bovine vasopressin and oxytocin precursor genes suggested by nucleotide sequence. Nature 308, 554-557.
  • Land H., Grez, M., Ruppert, S., Schmale, H., Rehbein, M., Richter, D. and Schütz, G. (1983). Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA. Nature 302, 342-344.

As a scientist at the renowned University of California at Berkeley and at Genentech, Inc., the first biotech company in the U.S., Dr. Ruppert also gained his first experience with U.S. patents.  Here, his scientific work, among others, led to three U.S. patents.

U.S. Patents

Dr. Ruppert’s scientific work at the University of California at Berkeley and at the U.S. biotech company Genentech, Inc. led, among other things, to the U.S. patents listed below:

  • Ruppert, S., (1999) United States Patent No. 5,891,637 “Construction of full-length cDNA libraries.”
  • Tjian, R., Comai, L., Dynlacht, B.D., Hoey, T., Ruppert, S., Tanese, N., Wang, E., Weinzierl, R.O.J. (1997) United States Patent No. 5,637,686 “TATA-binding protein associated factors, nucleic acids.”
  • Tjian, R., Comai, L., Dynlacht, B.D., Hoey, T., Ruppert, S., Tanese, N., Wang, E., Weinzierl, R.O.J. (1996) United States Patent No. 5,534,410 “TATA-binding protein associated factors drug screens.”